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95. Solving a chemistry problem
Content: 3[.docx 17,03 kB
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13(3). A weighed sample of m g of the natural product was dissolved in V ml of alcohol and 0.05 of the solution was applied to paper. After chromatographic separation, the obtained spots of uridylic acid, ribose-1,5-diphosphate and adenylic acid were cut out, fused with KOH and K2S2O8; after dissolving the fusion, phosphorus was determined photometrically, obtaining the optical density values of Aurid, Arib and Aadenyl, respectively; a solution with an optical density of Ast was prepared from a standard phosphate solution containing 20 μg P/ml. Determine the mass fraction (%) of these derivatives in the natural product.
Problem No. m, g V, ml Aurid Arib Aadenyl Ast
3 1.050 100 0.58 0.79 0.23 0.95
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