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82. Solving a chemistry problem
Content: 332.rar 49,63 kB
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332. The bean mosaic virus was investigated by centrifugation in an aqueous medium at 20 °C and the centrifuge rotor speed of 12590 min-1, and the following data were obtained on the distance of the sedimentation boundary from the axis of rotation of the rotor depending on the sedimentation time:
t, min 16 32 48 64 80 96 112
x*, cm 6.22 6.32 6.42 6.52 6.62 6.72 6.82
x* is the distance of the sedimentation boundary from the axis of rotation.
Based on these data, determine the sedimentation coefficient and the molar mass of the virus, if it is known that the diffusion coefficient is 0.51 * 10-11 m2 / s and the ascent coefficient (1 - Vsp * ρ0) is 0.268
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